Generation and characterization of a single-chain fv antibody against gli3, a hedgehog signaling pathway transcription factor.
Hybridoma (Larchmt). 2008 Jun;27(3):167-74
Authors: Laht S, Meerits K, Altroff H, Faust H, Tsaney R, Kogerman P, Järvekülg L, Paalme V, Valkna A, Timmusk S
Abstract Gli3 is a key regulator of development, controlling multiple patterning steps. Here we report the generation of a scFv antibody specific to the repressor domain of human Gli3. We show that this scFv retains the binding capacity of its parent anti-Gli3 monoclonal antibody derived from hybridoma clone 5E1. When expressed in mammalian cells, the anti-Gli3 scFv co-localizes with intracellular Gli3. Immunocytochemical staining of the intrabody in Gli3-positive TM4 cells shows a distinct perinuclear cytoplasmic localization. Such a scFv constitutes a useful tool for studying transcriptional regulation of the hedgehog pathway in mammals and offers a starting point for developing novel Gli-related therapeutic intrabodies.
PMID: 18582209 [PubMed - in process]
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Source:
PubMed: hedgehog
NCBI: db=PubMed; Term=hedgehog
